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enzyme linked immunospot elispot assay  (Cellular Technology Ltd)


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    Structured Review

    Cellular Technology Ltd enzyme linked immunospot elispot assay
    In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed <t>by</t> <t>enzyme-linked</t> immunospot <t>(ELISpot)</t> assay. Data were shown as mean ± SD (n = 3).
    Enzyme Linked Immunospot Elispot Assay, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 96/100, based on 929 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elispot+enzyme+linked+immunospot/Human+IFN-%CE%B3+Single-Color+ELISPOT/pmc12926576-337-88-89
    Average 96 stars, based on 929 article reviews
    enzyme linked immunospot elispot assay - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy"

    Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.02.018

    In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. Data were shown as mean ± SD (n = 3).
    Figure Legend Snippet: In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. Data were shown as mean ± SD (n = 3).

    Techniques Used: In Vivo, Activation Assay, Injection, Flow Cytometry, Enzyme-linked Immunospot

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Induction of cross-protection against influenza A virus by DNA prime-intranasal protein boost strategy based on nucleoprotein
    Article Snippet: .. Ab: Antibody; BSA: Bovine serum albumin; ELISA: Enzyme-linked immunosorbent assay; ELISpot: Enzyme-linked immunospot; CTL: Cytotoxic T cell; LD 50 : 50% lethal dose; NP: Nucleoprotein; M1: Matrix protein-1; M2: Matrix protein-2; PBS: Phosphate buffered saline; SPF: Sspecific pathogen free; TCID 50 : 50% tissue culture infection dose. ..

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    Article Title: Genetic diversity and circulation of influenza A viruses in Italian pig farms: insights from surveillance and vaccination.
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    Article Snippet: .. Abbreviations BSA: Bovine serum albumin; CTL: Cytotoxic T lymphocyte; ELISA: Enzymelinked immunosorbent assay; ELISPOT: Enzyme-linked immunospot; HA: Hemagglutinin; M1: Matrix protein 1; MLD50: 50% lethal dose in mice; NP: Nucleoprotein; PB1: Polymerase basic 1; PBS: Phosphate buffered saline; SFC: Spot Forming Cell; TCID50: 50% tissue culture infection dose. ..

    Enzyme-linked Immunospot:

    Article Title: Induction of cross-protection against influenza A virus by DNA prime-intranasal protein boost strategy based on nucleoprotein
    Article Snippet: .. Ab: Antibody; BSA: Bovine serum albumin; ELISA: Enzyme-linked immunosorbent assay; ELISpot: Enzyme-linked immunospot; CTL: Cytotoxic T cell; LD 50 : 50% lethal dose; NP: Nucleoprotein; M1: Matrix protein-1; M2: Matrix protein-2; PBS: Phosphate buffered saline; SPF: Sspecific pathogen free; TCID 50 : 50% tissue culture infection dose. ..

    Article Title: Strategies for developing multi-epitope subunit vaccines against tick-borne protozoa and bacteria.
    Article Snippet: The emergence of tick-borne protozoa and bacteria poses significant threats to both livestock and human health, underscoring the urgent need for effective vaccination strategies.. Multi-epitope subunit vaccines offer a promising solution, as they can elicit robust humoral and cellular immune responses.. This review synthesizes current knowledge on the development of multi-epitope subunit vaccines against tick-borne protozoan and bacterial pathogens.

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    Saline:

    Article Title: Induction of cross-protection against influenza A virus by DNA prime-intranasal protein boost strategy based on nucleoprotein
    Article Snippet: .. Ab: Antibody; BSA: Bovine serum albumin; ELISA: Enzyme-linked immunosorbent assay; ELISpot: Enzyme-linked immunospot; CTL: Cytotoxic T cell; LD 50 : 50% lethal dose; NP: Nucleoprotein; M1: Matrix protein-1; M2: Matrix protein-2; PBS: Phosphate buffered saline; SPF: Sspecific pathogen free; TCID 50 : 50% tissue culture infection dose. ..

    Article Title: Induction of cross-protection against influenza A virus by DNA prime-intranasal protein boost strategy based on nucleoprotein
    Article Snippet: .. Abbreviations Ab: Antibody; BSA: Bovine serum albumin; ELISA: Enzyme-linked immunosorbent assay; ELISpot: Enzyme-linked immunospot; CTL: Cytotoxic T cell; LD50: 50% lethal dose; NP: Nucleoprotein; M1: Matrix protein-1; M2: Matrix protein-2; PBS: Phosphate buffered saline; SPF: Sspecific pathogen free; TCID50: 50% tissue culture infection dose. ..

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    Article Title: Influenza A virus nucleoprotein derived from Escherichia coli or recombinant vaccinia (Tiantan) virus elicits robust cross-protection in mice
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    Infection:

    Article Title: Induction of cross-protection against influenza A virus by DNA prime-intranasal protein boost strategy based on nucleoprotein
    Article Snippet: .. Ab: Antibody; BSA: Bovine serum albumin; ELISA: Enzyme-linked immunosorbent assay; ELISpot: Enzyme-linked immunospot; CTL: Cytotoxic T cell; LD 50 : 50% lethal dose; NP: Nucleoprotein; M1: Matrix protein-1; M2: Matrix protein-2; PBS: Phosphate buffered saline; SPF: Sspecific pathogen free; TCID 50 : 50% tissue culture infection dose. ..

    Article Title: Induction of cross-protection against influenza A virus by DNA prime-intranasal protein boost strategy based on nucleoprotein
    Article Snippet: .. Abbreviations Ab: Antibody; BSA: Bovine serum albumin; ELISA: Enzyme-linked immunosorbent assay; ELISpot: Enzyme-linked immunospot; CTL: Cytotoxic T cell; LD50: 50% lethal dose; NP: Nucleoprotein; M1: Matrix protein-1; M2: Matrix protein-2; PBS: Phosphate buffered saline; SPF: Sspecific pathogen free; TCID50: 50% tissue culture infection dose. ..

    Article Title: Construction and immune effect of an HPV16/18/58 trivalent therapeutic adenovirus vector vaccine.
    Article Snippet: .. Abbreviations HPV: Human papillomaviruses; AD: Adenovirus-negative control; NC: Negative control; CTL: Cytotoxic T lymphocyte; RT-PCR: Reverse transcription-polymerase chain reaction; PBS: Phosphate buffered saline; VLP: Virus like particle; HLA: Human leukocyte antigen; CPE: Cytopathic effect; MOI: Multiple of infection; PFU: Plaque forming unit; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; LV: Lentivirus; ELISA: Enzyme-linked immunosorbent assay; ELISPOT: Enzyme-linked immunospot; OD: Optical density; PMA: Phorbol-12-myristate13-acetate; LDH: Lactate dehydrogenase; SPSS: Statistical product and service solutions; RCA : Replication-deficient adenovirus; HSP: Heat shock protein; GM-CSF: Granulocyte macrophage-colony stimulating factor. ..

    Article Title: Influenza A virus nucleoprotein derived from Escherichia coli or recombinant vaccinia (Tiantan) virus elicits robust cross-protection in mice
    Article Snippet: .. Abbreviations BSA: Bovine serum albumin; CTL: Cytotoxic T lymphocyte; ELISA: Enzymelinked immunosorbent assay; ELISPOT: Enzyme-linked immunospot; HA: Hemagglutinin; M1: Matrix protein 1; MLD50: 50% lethal dose in mice; NP: Nucleoprotein; PB1: Polymerase basic 1; PBS: Phosphate buffered saline; SFC: Spot Forming Cell; TCID50: 50% tissue culture infection dose. ..

    Immunopeptidomics:

    Article Title: Strategies for developing multi-epitope subunit vaccines against tick-borne protozoa and bacteria.
    Article Snippet: The emergence of tick-borne protozoa and bacteria poses significant threats to both livestock and human health, underscoring the urgent need for effective vaccination strategies.. Multi-epitope subunit vaccines offer a promising solution, as they can elicit robust humoral and cellular immune responses.. This review synthesizes current knowledge on the development of multi-epitope subunit vaccines against tick-borne protozoan and bacterial pathogens.

    Software:

    Article Title: Strategies for developing multi-epitope subunit vaccines against tick-borne protozoa and bacteria.
    Article Snippet: The emergence of tick-borne protozoa and bacteria poses significant threats to both livestock and human health, underscoring the urgent need for effective vaccination strategies.. Multi-epitope subunit vaccines offer a promising solution, as they can elicit robust humoral and cellular immune responses.. This review synthesizes current knowledge on the development of multi-epitope subunit vaccines against tick-borne protozoan and bacterial pathogens.

    Reverse Transcription:

    Article Title: Strategies for developing multi-epitope subunit vaccines against tick-borne protozoa and bacteria.
    Article Snippet: The emergence of tick-borne protozoa and bacteria poses significant threats to both livestock and human health, underscoring the urgent need for effective vaccination strategies.. Multi-epitope subunit vaccines offer a promising solution, as they can elicit robust humoral and cellular immune responses.. This review synthesizes current knowledge on the development of multi-epitope subunit vaccines against tick-borne protozoan and bacterial pathogens.

    Real-time Polymerase Chain Reaction:

    Article Title: Strategies for developing multi-epitope subunit vaccines against tick-borne protozoa and bacteria.
    Article Snippet: The emergence of tick-borne protozoa and bacteria poses significant threats to both livestock and human health, underscoring the urgent need for effective vaccination strategies.. Multi-epitope subunit vaccines offer a promising solution, as they can elicit robust humoral and cellular immune responses.. This review synthesizes current knowledge on the development of multi-epitope subunit vaccines against tick-borne protozoan and bacterial pathogens.

    Control:

    Article Title: Construction and immune effect of an HPV16/18/58 trivalent therapeutic adenovirus vector vaccine.
    Article Snippet: .. Abbreviations HPV: Human papillomaviruses; AD: Adenovirus-negative control; NC: Negative control; CTL: Cytotoxic T lymphocyte; RT-PCR: Reverse transcription-polymerase chain reaction; PBS: Phosphate buffered saline; VLP: Virus like particle; HLA: Human leukocyte antigen; CPE: Cytopathic effect; MOI: Multiple of infection; PFU: Plaque forming unit; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; LV: Lentivirus; ELISA: Enzyme-linked immunosorbent assay; ELISPOT: Enzyme-linked immunospot; OD: Optical density; PMA: Phorbol-12-myristate13-acetate; LDH: Lactate dehydrogenase; SPSS: Statistical product and service solutions; RCA : Replication-deficient adenovirus; HSP: Heat shock protein; GM-CSF: Granulocyte macrophage-colony stimulating factor. ..

    Negative Control:

    Article Title: Construction and immune effect of an HPV16/18/58 trivalent therapeutic adenovirus vector vaccine.
    Article Snippet: .. Abbreviations HPV: Human papillomaviruses; AD: Adenovirus-negative control; NC: Negative control; CTL: Cytotoxic T lymphocyte; RT-PCR: Reverse transcription-polymerase chain reaction; PBS: Phosphate buffered saline; VLP: Virus like particle; HLA: Human leukocyte antigen; CPE: Cytopathic effect; MOI: Multiple of infection; PFU: Plaque forming unit; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; LV: Lentivirus; ELISA: Enzyme-linked immunosorbent assay; ELISPOT: Enzyme-linked immunospot; OD: Optical density; PMA: Phorbol-12-myristate13-acetate; LDH: Lactate dehydrogenase; SPSS: Statistical product and service solutions; RCA : Replication-deficient adenovirus; HSP: Heat shock protein; GM-CSF: Granulocyte macrophage-colony stimulating factor. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Construction and immune effect of an HPV16/18/58 trivalent therapeutic adenovirus vector vaccine.
    Article Snippet: .. Abbreviations HPV: Human papillomaviruses; AD: Adenovirus-negative control; NC: Negative control; CTL: Cytotoxic T lymphocyte; RT-PCR: Reverse transcription-polymerase chain reaction; PBS: Phosphate buffered saline; VLP: Virus like particle; HLA: Human leukocyte antigen; CPE: Cytopathic effect; MOI: Multiple of infection; PFU: Plaque forming unit; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; LV: Lentivirus; ELISA: Enzyme-linked immunosorbent assay; ELISPOT: Enzyme-linked immunospot; OD: Optical density; PMA: Phorbol-12-myristate13-acetate; LDH: Lactate dehydrogenase; SPSS: Statistical product and service solutions; RCA : Replication-deficient adenovirus; HSP: Heat shock protein; GM-CSF: Granulocyte macrophage-colony stimulating factor. ..

    Virus:

    Article Title: Construction and immune effect of an HPV16/18/58 trivalent therapeutic adenovirus vector vaccine.
    Article Snippet: .. Abbreviations HPV: Human papillomaviruses; AD: Adenovirus-negative control; NC: Negative control; CTL: Cytotoxic T lymphocyte; RT-PCR: Reverse transcription-polymerase chain reaction; PBS: Phosphate buffered saline; VLP: Virus like particle; HLA: Human leukocyte antigen; CPE: Cytopathic effect; MOI: Multiple of infection; PFU: Plaque forming unit; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; LV: Lentivirus; ELISA: Enzyme-linked immunosorbent assay; ELISPOT: Enzyme-linked immunospot; OD: Optical density; PMA: Phorbol-12-myristate13-acetate; LDH: Lactate dehydrogenase; SPSS: Statistical product and service solutions; RCA : Replication-deficient adenovirus; HSP: Heat shock protein; GM-CSF: Granulocyte macrophage-colony stimulating factor. ..

    HI Assay:

    Article Title: Genetic diversity and circulation of influenza A viruses in Italian pig farms: insights from surveillance and vaccination.
    Article Snippet: .. NP ELISA: Nucleoprotein ELISA; HI: hemagglutination inhibition; ELISPOT: Enzyme-Linked ImmunoSpot. ..



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    Cellular Technology Ltd t cell enzyme linked cytokine immunospot elispot assays
    In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed <t>by</t> <t>enzyme-linked</t> immunospot <t>(ELISpot)</t> assay. Data were shown as mean ± SD (n = 3).
    T Cell Enzyme Linked Cytokine Immunospot Elispot Assays, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    t cell enzyme linked cytokine immunospot elispot assays - by Bioz Stars, 2026-09
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    In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. Data were shown as mean ± SD (n = 3).

    Journal: Bioactive Materials

    Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy

    doi: 10.1016/j.bioactmat.2026.02.018

    Figure Lengend Snippet: In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. Data were shown as mean ± SD (n = 3).

    Article Snippet: Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay.

    Techniques: In Vivo, Activation Assay, Injection, Flow Cytometry, Enzyme-linked Immunospot